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Created as a high-throughput facility to artificially-rear newborn piglets and conduct behavioral testing, this unique infrastructure is designed to investigate how early-life nutrition influences growth and development of the brain. With an experienced facility coordinator and capacity to raise 48 newborn piglets simultaneously, PNCL offers collaborative opportunities to use the piglet as a pre-clinical model in biomedical research. A dedicated behavioral suite, seperate from the piglet rearing room, permits sensitive testing of eyeblink conditioning and novelty preference paradigms. A custom-designed automated feeding system ensures precise delivery of dietary treatments to individual subjects. Home-cage video provides 24/7 monitoring of piglet behavior, allowing for characterization of sleep and activity patterns. This research area is focused on studying the interaction of early-life nutrition on development of a stable microbiota, immunity, and gut and brain function.